E-ISSN 0331-8753 | ISSN 0794-5698
 

Original Research 


Stability Profile of Pistia stratiotes Leaf Peroxidase

Rotimi Olusanya Arise, Bolaji O. Osundahunsi.

Abstract
Peroxidases are widely distributed in nature and are involved in multiple industrial applications owing to their critical physiological roles. Sources of peroxidases with notable stability are therefore being explored for commercial purposes. Pistia stratiotes leaf peroxidase was purified and characterized for its stability properties. Its optimum pH shifted from 6.0 to 6.5 after incubation for 1 hour while it showed moderate stability after heating at 50˚C for 60 minutes and at 60˚C for 30 minutes. P. stratiotes leaf peroxidase was strongly inhibited by EDTA while urea had only a slight denaturing effect with approximately 65% of the activity retained after 60 minutes exposure. There was reduction in P. stratiotes leaf peroxidase activity in the presence of Pb2+, Hg2+, Ni2+, Cu2+, Co2+, Ca2+ and Mn2+. Acetone activated the enzyme in a concentration-dependent manner while the presence of methanol and ethanol in the reaction mixture led to decrease in the activity of the enzyme. Results obtained from this study revealed that P. stratiotes leaf peroxidase is moderately stable and thus has potential for some industrial applications.

Key words: Pistia stratiotes, urea, enzyme activity, peroxidase, stability


 
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How to Cite this Article
Pubmed Style

Arise RO, Osundahunsi BO. Stability Profile of Pistia stratiotes Leaf Peroxidase. Nig. J. Basic Appl. Sci.. 2018; 26(1): 96-105.


Web Style

Arise RO, Osundahunsi BO. Stability Profile of Pistia stratiotes Leaf Peroxidase. www.njbas-udus.com/?mno=230059 [Access: August 19, 2019].


AMA (American Medical Association) Style

Arise RO, Osundahunsi BO. Stability Profile of Pistia stratiotes Leaf Peroxidase. Nig. J. Basic Appl. Sci.. 2018; 26(1): 96-105.



Vancouver/ICMJE Style

Arise RO, Osundahunsi BO. Stability Profile of Pistia stratiotes Leaf Peroxidase. Nig. J. Basic Appl. Sci.. (2018), [cited August 19, 2019]; 26(1): 96-105.



Harvard Style

Arise, R. O. & Osundahunsi, . B. O. (2018) Stability Profile of Pistia stratiotes Leaf Peroxidase. Nig. J. Basic Appl. Sci., 26 (1), 96-105.



Turabian Style

Arise, Rotimi Olusanya, and Bolaji O. Osundahunsi. 2018. Stability Profile of Pistia stratiotes Leaf Peroxidase. Nigerian Journal of Basic and Applied Sciences, 26 (1), 96-105.



Chicago Style

Arise, Rotimi Olusanya, and Bolaji O. Osundahunsi. "Stability Profile of Pistia stratiotes Leaf Peroxidase." Nigerian Journal of Basic and Applied Sciences 26 (2018), 96-105.



MLA (The Modern Language Association) Style

Arise, Rotimi Olusanya, and Bolaji O. Osundahunsi. "Stability Profile of Pistia stratiotes Leaf Peroxidase." Nigerian Journal of Basic and Applied Sciences 26.1 (2018), 96-105. Print.



APA (American Psychological Association) Style

Arise, R. O. & Osundahunsi, . B. O. (2018) Stability Profile of Pistia stratiotes Leaf Peroxidase. Nigerian Journal of Basic and Applied Sciences, 26 (1), 96-105.