ISSN 0331-8753
 

Original Research 


Stability Profile of Pistia stratiotes Leaf Peroxidase

Rotimi Olusanya Arise, Bolaji O. Osundahunsi.

Abstract
Peroxidases are widely distributed in nature and are involved in multiple industrial applications owing to their critical physiological roles. Sources of peroxidases with notable stability are therefore being explored for commercial purposes. Pistia stratiotes leaf peroxidase was purified and characterized for its stability properties. Its optimum pH shifted from 6.0 to 6.5 after incubation for 1 hour while it showed moderate stability after heating at 50˚C for 60 minutes and at 60˚C for 30 minutes. P. stratiotes leaf peroxidase was strongly inhibited by EDTA while urea had only a slight denaturing effect with approximately 65% of the activity retained after 60 minutes exposure. There was reduction in P. stratiotes leaf peroxidase activity in the presence of Pb2+, Hg2+, Ni2+, Cu2+, Co2+, Ca2+ and Mn2+. Acetone activated the enzyme in a concentration-dependent manner while the presence of methanol and ethanol in the reaction mixture led to decrease in the activity of the enzyme. Results obtained from this study revealed that P. stratiotes leaf peroxidase is moderately stable and thus has potential for some industrial applications.

Key words: Pistia stratiotes, urea, enzyme activity, peroxidase, stability


 
ARTICLE TOOLS
Abstract
PDF Fulltext
Print this article Print this Article
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Rotimi Olusanya Arise
Articles by Bolaji O. Osundahunsi
on Google
on Google Scholar
Article Statistics
 Viewed: 34
Downloaded: 44
Cited: 0

How to Cite this Article
Pubmed Style

Arise RO, Osundahunsi BO. Stability Profile of Pistia stratiotes Leaf Peroxidase. Nig. J. Basic Appl. Sci.. 2018; 26(1): 96-105.


Web Style

Arise RO, Osundahunsi BO. Stability Profile of Pistia stratiotes Leaf Peroxidase. http://www.njbas-udus.com/?mno=230059 [Access: December 14, 2018].


AMA (American Medical Association) Style

Arise RO, Osundahunsi BO. Stability Profile of Pistia stratiotes Leaf Peroxidase. Nig. J. Basic Appl. Sci.. 2018; 26(1): 96-105.



Vancouver/ICMJE Style

Arise RO, Osundahunsi BO. Stability Profile of Pistia stratiotes Leaf Peroxidase. Nig. J. Basic Appl. Sci.. (2018), [cited December 14, 2018]; 26(1): 96-105.



Harvard Style

Arise, R. O. & Osundahunsi, . B. O. (2018) Stability Profile of Pistia stratiotes Leaf Peroxidase. Nig. J. Basic Appl. Sci., 26 (1), 96-105.



Turabian Style

Arise, Rotimi Olusanya, and Bolaji O. Osundahunsi. 2018. Stability Profile of Pistia stratiotes Leaf Peroxidase. Nigerian Journal of Basic and Applied Sciences, 26 (1), 96-105.



Chicago Style

Arise, Rotimi Olusanya, and Bolaji O. Osundahunsi. "Stability Profile of Pistia stratiotes Leaf Peroxidase." Nigerian Journal of Basic and Applied Sciences 26 (2018), 96-105.



MLA (The Modern Language Association) Style

Arise, Rotimi Olusanya, and Bolaji O. Osundahunsi. "Stability Profile of Pistia stratiotes Leaf Peroxidase." Nigerian Journal of Basic and Applied Sciences 26.1 (2018), 96-105. Print.



APA (American Psychological Association) Style

Arise, R. O. & Osundahunsi, . B. O. (2018) Stability Profile of Pistia stratiotes Leaf Peroxidase. Nigerian Journal of Basic and Applied Sciences, 26 (1), 96-105.





Most Viewed Articles
  • Isolation and Identification of Fungi Associated with the Spoilage of Sweet Orange (Citrus Sinensis) Fruits In Sokoto State
    I.Y. Tafinta, K.Shehu, H. Abdulganiyyu, A.M. Rabe and A. Usman
    Nig. J. Basic Appl. Sci.. 2013; 21(3): 193-196
    » Abstract & References

  • Heavy Metals and Physicochemical Properties of Soils in Kano Urban Agricultural Lands
    U.M. Dawaki, A.U. Dikko, S.S. Noma and U. Aliyu
    Nig. J. Basic Appl. Sci.. 2013; 21(3): 239-246
    » Abstract & References

  • Study on Biochemical Indices of Liver Function Tests of Albino Rats Supplemented with Three Sources of Vegetable Oils [English]
    K.E. Imafidon and L.O. Okunrobo
    Nig. J. Basic Appl. Sci.. 2012; 20(2): 105-110
    » Abstract

  • Effect of Sources of Organic Manure on Growth and Yields of Okra (Abelmoschus esculentus L.) in Sokoto, Nigeria [English]
    R.A. Tiamiyu, H.G. Ahmed and A.S. Muhammad
    Nig. J. Basic Appl. Sci.. 2012; 20(3): 213-216
    » Abstract

  • Optimum Temperature and Thermal Stability of Crude Polyphenol Oxidase from some Common Fruits
    AB Bello, MS Sule
    Nig. J. Basic Appl. Sci.. 2012; 20(1): 27-31
    » Abstract

  • Effect of Camel Milks Supplementation on Serum Glucose Levels, Lipid Profile and Body Weight of Alloxan-Induced Diabetic Rats
    S.A. Isa, K.G. Ibrahim and I. Abubakar
    Nig. J. Basic Appl. Sci.. 2013; 21(3): 187-192
    » Abstract & References

  • Gonadosomatic Index, Fecundity and Egg Size of Auchenoglanis occidentalis (Cuvier and Valenciennes) in River Rima, North-Western Nigeria [English]
    B.A. Shinkafi and J.K. Ipinjolu
    Nig. J. Basic Appl. Sci.. 2012; 20(3): 217-224
    » Abstract

  • Chemical Composition of Kapok (Ceibapentandra) Seed and Physicochemical Properties of its Oil [English]
    K.M. Anigo,B.M.D. Dauda,A.B. Sallau and I.E.Chindo
    Nig. J. Basic Appl. Sci.. 2013; 21(2): 105-108
    » Abstract

  • Biogas production through Co-digestion of palm oil mill effluent with cow manure
    Umar Hayatu Sidik, Firdausi Bin Razali, Sharifah Rafidah Wan Alwi, Fadimtu Maigari
    Nig. J. Basic Appl. Sci.. 2013; 21(1): 79-84
    » Abstract

  • Morphological Assessment of the Genetic Variability among 53 Accessions of West African okra [Abelmoschus caillei (A. Chev.) Stevels] from South Western Nigeria
    M.E. Osawaru, M.C. Ogwu and F.M. Dania-Ogbe
    Nig. J. Basic Appl. Sci.. 2013; 21(3): 227-238
    » Abstract & References

  • Most Downloaded
  • Isolation and Identification of Fungi Associated with the Spoilage of Sweet Orange (Citrus Sinensis) Fruits In Sokoto State
    I.Y. Tafinta, K.Shehu, H. Abdulganiyyu, A.M. Rabe and A. Usman
    Nig. J. Basic Appl. Sci.. 2013; 21(3): 193-196
    » Abstract & References

  • Heavy Metals and Physicochemical Properties of Soils in Kano Urban Agricultural Lands
    U.M. Dawaki, A.U. Dikko, S.S. Noma and U. Aliyu
    Nig. J. Basic Appl. Sci.. 2013; 21(3): 239-246
    » Abstract & References

  • Optimization of Steam Distillation of Essential Oil of Eucalyptus tereticornis by Response Surface Methodology [English]
    M.S. Galadima, A.S. Ahmed, A.S. Olawale and I. M. Bugaje
    Nig. J. Basic Appl. Sci.. 2012; 20(4): 368-372
    » Abstract

  • Study on Biochemical Indices of Liver Function Tests of Albino Rats Supplemented with Three Sources of Vegetable Oils [English]
    K.E. Imafidon and L.O. Okunrobo
    Nig. J. Basic Appl. Sci.. 2012; 20(2): 105-110
    » Abstract

  • Effect of Camel Milks Supplementation on Serum Glucose Levels, Lipid Profile and Body Weight of Alloxan-Induced Diabetic Rats
    S.A. Isa, K.G. Ibrahim and I. Abubakar
    Nig. J. Basic Appl. Sci.. 2013; 21(3): 187-192
    » Abstract & References

  • Biogas production through Co-digestion of palm oil mill effluent with cow manure
    Umar Hayatu Sidik, Firdausi Bin Razali, Sharifah Rafidah Wan Alwi, Fadimtu Maigari
    Nig. J. Basic Appl. Sci.. 2013; 21(1): 79-84
    » Abstract

  • Gonadosomatic Index, Fecundity and Egg Size of Auchenoglanis occidentalis (Cuvier and Valenciennes) in River Rima, North-Western Nigeria [English]
    B.A. Shinkafi and J.K. Ipinjolu
    Nig. J. Basic Appl. Sci.. 2012; 20(3): 217-224
    » Abstract

  • Antibacterial and Anti-Inflammatory Activities of Anacardium occidentale Leaves and Bark Extracts
    B.T. Thomas, M.O. Soladoye, T.T. Adegboyega, G.C. Agu and O.D. Popoola
    Nig. J. Basic Appl. Sci.. 2015; 23(1): 1-6
    » Abstract

  • The Swelling Behaviour of Polystyrene (PS)/ Polyvinylacetate (Pvac) Blends in Different Solvents and the Effects of -Cellulose used as Filler on the Electrical Conductivity. [English]
    P. A. P. Mamza and B.T. Nwufo
    Nig. J. Basic Appl. Sci.. 2008; 16(2): 165 -
    » Abstract

  • Morphological Assessment of the Genetic Variability among 53 Accessions of West African okra [Abelmoschus caillei (A. Chev.) Stevels] from South Western Nigeria
    M.E. Osawaru, M.C. Ogwu and F.M. Dania-Ogbe
    Nig. J. Basic Appl. Sci.. 2013; 21(3): 227-238
    » Abstract & References

  • Most Cited Articles