E-ISSN 0331-8753 | ISSN 0794-5698
 

Original Research 


Expression of Wild-Type p53 by Curcumin, Alpinetin and Flavokawain B in Colorectal Cancer cells Expressing R273H Mutant p53

I. Malami, A. Muhammad, I. B. Abubakar and A. M. Alhassan.

Abstract
A mutation in p53 is frequently reported in nearly 50% of all of human cancers arising from DNA-binding core domain of p53. DNA-contact mutant R273H rendered p53 at dysfunctional state due to the substitution of single residue Arg273 for His273. Here, natural bioactive compounds curcumin, alpinetin and flavokawain B were investigated for possible stabilisation of wild-type p53 expression in vitro using HT-29 cells harbouring R273H rendered p53. Accordingly, all the bioactive compounds were able to induce the expression of wild-type p53 both at the levels of gene and protein expression. A dose dependent induction of p53 was evident at 12.5, 25 and 50 M concentration. The present study have substantiate evidences that the bioactive compounds may have restored the wild-type p53 functional activity in tumour cells expressing R273H mutant p53.

Key words: Curcumin, Alpinetin, Flavokawain B, p53, R273H


 
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How to Cite this Article
Pubmed Style

Malami I, Muhammad A, . Expression of Wild-Type p53 by Curcumin, Alpinetin and Flavokawain B in Colorectal Cancer cells Expressing R273H Mutant p53. Nig. J. Basic Appl. Sci.. 2019; 27(2): 88-94. doi:10.4314/njbas.v27i2.12


Web Style

Malami I, Muhammad A, . Expression of Wild-Type p53 by Curcumin, Alpinetin and Flavokawain B in Colorectal Cancer cells Expressing R273H Mutant p53. http://www.njbas-udus.com/?mno=42251 [Access: March 28, 2020]. doi:10.4314/njbas.v27i2.12


AMA (American Medical Association) Style

Malami I, Muhammad A, . Expression of Wild-Type p53 by Curcumin, Alpinetin and Flavokawain B in Colorectal Cancer cells Expressing R273H Mutant p53. Nig. J. Basic Appl. Sci.. 2019; 27(2): 88-94. doi:10.4314/njbas.v27i2.12



Vancouver/ICMJE Style

Malami I, Muhammad A, . Expression of Wild-Type p53 by Curcumin, Alpinetin and Flavokawain B in Colorectal Cancer cells Expressing R273H Mutant p53. Nig. J. Basic Appl. Sci.. (2019), [cited March 28, 2020]; 27(2): 88-94. doi:10.4314/njbas.v27i2.12



Harvard Style

Malami, I., Muhammad, . A. & (2019) Expression of Wild-Type p53 by Curcumin, Alpinetin and Flavokawain B in Colorectal Cancer cells Expressing R273H Mutant p53. Nig. J. Basic Appl. Sci., 27 (2), 88-94. doi:10.4314/njbas.v27i2.12



Turabian Style

Malami, I., A. Muhammad, and I. B. Abubakar and A. M. Alhassan. 2019. Expression of Wild-Type p53 by Curcumin, Alpinetin and Flavokawain B in Colorectal Cancer cells Expressing R273H Mutant p53. Nigerian Journal of Basic and Applied Sciences, 27 (2), 88-94. doi:10.4314/njbas.v27i2.12



Chicago Style

Malami, I., A. Muhammad, and I. B. Abubakar and A. M. Alhassan. "Expression of Wild-Type p53 by Curcumin, Alpinetin and Flavokawain B in Colorectal Cancer cells Expressing R273H Mutant p53." Nigerian Journal of Basic and Applied Sciences 27 (2019), 88-94. doi:10.4314/njbas.v27i2.12



MLA (The Modern Language Association) Style

Malami, I., A. Muhammad, and I. B. Abubakar and A. M. Alhassan. "Expression of Wild-Type p53 by Curcumin, Alpinetin and Flavokawain B in Colorectal Cancer cells Expressing R273H Mutant p53." Nigerian Journal of Basic and Applied Sciences 27.2 (2019), 88-94. Print. doi:10.4314/njbas.v27i2.12



APA (American Psychological Association) Style

Malami, I., Muhammad, . A. & (2019) Expression of Wild-Type p53 by Curcumin, Alpinetin and Flavokawain B in Colorectal Cancer cells Expressing R273H Mutant p53. Nigerian Journal of Basic and Applied Sciences, 27 (2), 88-94. doi:10.4314/njbas.v27i2.12





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